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viral packaging envelope plasmids pmd2g  (Addgene inc)


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    Structured Review

    Addgene inc viral packaging envelope plasmids pmd2g
    Viral Packaging Envelope Plasmids Pmd2g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viral+packaging+envelope+plasmids+pmd2g/pMD2%2EG+(Plasmid+%2312259)/pmc12032931-434-23-28
    Average 98 stars, based on 14770 article reviews
    viral packaging envelope plasmids pmd2g - by Bioz Stars, 2026-09
    98/100 stars

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    Produced:

    Article Title: Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liver
    Article Snippet: MC38 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml Penicillin, 100 μg/ml Streptomycin (Sigma-Aldrich) and 2 mM L-Glutamine (Gibco). mCherry-expressing APTKA (APTKA-mCherry), luciferase-expressing MC38 (MC38-luc) and luciferase-expressing APTKA (APTKA-luc) organoids were generated by lentiviral transduction using 8 μg/ml polybrene (Sigma-Aldrich). .. Lentiviruses were produced by co-transfection of the respective lentiviral expression vector pLV-mCherry (Addgene #36084) and pLenti PGK V5-LUC Neo (Addgene #21471) and the viral packaging envelope plasmids pMD2g (Addgene #12259) and pCMV-dR8.91 (provided by Christian Münz, UZH, Switzerland) into 293T cells (ATCC, CRL-3216). .. A DNA:polyethylenimine (Polysciences) μg ratio of 1:3 was used for transfection. mCherry + cells were sorted by Fluorescence-activated cell sorting (FACS).

    Article Title: Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liver
    Article Snippet: MC38 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml Penicillin, 100 μg/ml Streptomycin (Sigma-Aldrich) and 2 mM L-Glutamine (Gibco). mCherry-expressing APTKA (APTKA-mCherry), luciferase-expressing MC38 (MC38-luc), and luciferase-expressing APTKA (APTKA-luc) organoids were generated by lentiviral transduction using 8 μg/ml polybrene (Sigma-Aldrich). .. Lentiviruses were produced by co-transfection of the respective lentiviral expression vector pLV-mCherry (Addgene #36084) and pLenti PGK V5-LUC Neo (Addgene #21471) and the viral packaging envelope plasmids pMD2g (Addgene #12259) and pCMV-dR8.91 (provided by Christian Münz, UZH, Switzerland) into 293T cells (ATCC, CRL-3216). .. A DNA:polyethylenimine (Polysciences) μg ratio of 1:3 was used for transfection. mCherry + cells were sorted by Fluorescence-Activated Cell Sorting (FACS).

    Expressing:

    Article Title: Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liver
    Article Snippet: MC38 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml Penicillin, 100 μg/ml Streptomycin (Sigma-Aldrich) and 2 mM L-Glutamine (Gibco). mCherry-expressing APTKA (APTKA-mCherry), luciferase-expressing MC38 (MC38-luc) and luciferase-expressing APTKA (APTKA-luc) organoids were generated by lentiviral transduction using 8 μg/ml polybrene (Sigma-Aldrich). .. Lentiviruses were produced by co-transfection of the respective lentiviral expression vector pLV-mCherry (Addgene #36084) and pLenti PGK V5-LUC Neo (Addgene #21471) and the viral packaging envelope plasmids pMD2g (Addgene #12259) and pCMV-dR8.91 (provided by Christian Münz, UZH, Switzerland) into 293T cells (ATCC, CRL-3216). .. A DNA:polyethylenimine (Polysciences) μg ratio of 1:3 was used for transfection. mCherry + cells were sorted by Fluorescence-activated cell sorting (FACS).

    Article Title: Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liver
    Article Snippet: MC38 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml Penicillin, 100 μg/ml Streptomycin (Sigma-Aldrich) and 2 mM L-Glutamine (Gibco). mCherry-expressing APTKA (APTKA-mCherry), luciferase-expressing MC38 (MC38-luc), and luciferase-expressing APTKA (APTKA-luc) organoids were generated by lentiviral transduction using 8 μg/ml polybrene (Sigma-Aldrich). .. Lentiviruses were produced by co-transfection of the respective lentiviral expression vector pLV-mCherry (Addgene #36084) and pLenti PGK V5-LUC Neo (Addgene #21471) and the viral packaging envelope plasmids pMD2g (Addgene #12259) and pCMV-dR8.91 (provided by Christian Münz, UZH, Switzerland) into 293T cells (ATCC, CRL-3216). .. A DNA:polyethylenimine (Polysciences) μg ratio of 1:3 was used for transfection. mCherry + cells were sorted by Fluorescence-Activated Cell Sorting (FACS).



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    Addgene inc viral packaging envelope plasmids pmd2g
    Viral Packaging Envelope Plasmids Pmd2g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viral+packaging+envelope+plasmids+pmd2g/pMD2%2EG+(Plasmid+%2312259)/pmc12032931-434-23-28
    Average 98 stars, based on 1 article reviews
    viral packaging envelope plasmids pmd2g - by Bioz Stars, 2026-09
    98/100 stars
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